Antibacterial activity dental varnish prepared using titanium oxide nanoparticles using ginger and rosemary against Staphylococcus aureus and Enterococcus faecalis

Abstract

Nanomedicine, which combines medicine and nanotechnology, has a number of benefits over more conventional forms of therapy. Due to their distinctive
characteristics compared to their bulk equivalent, metal nanoparticles have increased tremendous attention in the field of nanomedicine. To study the mechanism
of action of rosemary and ginger-mediated titanium oxide nanoparticles (TiO2 NPs)-based dental varnish against Streptococcus mutans (S. mutans) and E.faecalis.
Materials and methods:MHA broth was prepared. Bacterial suspension (Staphylococcus aureus and MRSA) was added. The incubation is done under suitable
conditions for varied time intervals. Then the percentage of dead cells is calculated at a wavelength of 540 nm at regular time intervals. Result: The results have
demonstrated that when concentration rises, optical density values fall, demonstrating a bactericidal action. The results show a great difference between the values
of optical density of the test samples at various concentrations in the order of 25, 50, and 100 μL at various time intervals from 1hr, 2hr, 3hr, 4hr, 5hr being the
highest when compared with control and antibiotic groups against Streptococcus mutans and E.faecalis. The results have proved that the greenly generated dental
varnish has demonstrated good antibacterial and antibiofilm properties. Conclusion: From our study we concluded titanium oxide dental varnish prepared using
plant extract of rosemary and ginger showed considerable antibacterial activity against s. Aureus and e.faecalis