Phytosynthesis of Titanium oxides from Andrographis paniculata and its antibacterial efficacy against isolate S.mutans

Abstract

Andrographis paniculata Wall Acanthaceae family is a widely used medicinal herb with antimicrobial cytotoxic antiprotozoan anti inflammatory antioxidant immunostimulant antidiabetic and anti infective effects. Researchers are now mainly focused on synthesizing nanoparticles using eco friendly methods to enhance their biological efficacy. This species has pharmacological properties such as antibacterial antioxidant anti inflammatory antiparasitic antihyperglycemic hypoglycemic and antiallergic properties. Andrographis paniculata colonies were inoculated into Luria Bertani LB broth and incubated for 48 hours at 37 degrees Celsius. The solution was centrifuged at 4500 RPM for 15 minutes at 25 degrees Celsius. The pellets and supernatant were collected afterwards. The synthesis of titanium dioxide TiO2 nanoparticles showed antimicrobial activity against the oral pathogen Streptococcus mutans. Based on the findings further studies are needed to explore Andrographis paniculata mediated synthesis of TiO2 nanoparticles as a potential antibacterial oral mouthwash. In another study the antibacterial activity of synthesized TiO2 nanoparticles was evaluated using the agar well diffusion method. Fresh colonies of Bacillus species Escherichia coli and Salmonella species were used.