Frequency of genotypically confirmed VRSA and MRSA Among Children with Urinary Tract Infection.

Abstract

Background:-Urinary system Infections are common bacterial-infections in children. Staphylococcus Spp. especially Staphylococcus aureusmethicillin-resistant, (MRSA) strain is a important bacteria pathogen that causes many infections. Vancomycin-resistance Staphylococcus aureus
strain (VRSA) among “MRSA” isolates is an emerging threat associated with presumptive treatment of these infection-s. Therefore, the aims of
these study to determined the frequency of Staphylococcus aureus (S. aureus) isolates in children with UTI and detect mecA (methicillin
resistance) and vanA (Vancomycin resistance) genes among the isolates that responsible of various antimicrobial resistance. Methods:- A total
of (850) urine samples from the children that suspected to UTI were collected. of Staphylococcus aureus identified using culture methods and
biochemical tests then isolates confirmed by PCR (16S rRNA). MRSA screening was performed using the antibiotic cefoxitin disks and for
screening of VRSA using the vancomycin broth minimal inhibitory concentration (MIC).Then confirmed by PCR using mecA and vanA gene.
Results: A total of (265/850) Staphylococcus Spp. (31.2%) were isolates from patients had urinary-tract infection. Out of (265) Staphylococcus
Spp. (115) isolates was Staphylococcus aureus (43.4%) confirmed genotypically using (16S rRNA). A total of (96/115) S. aureus isolates (83.5%) determined
methicillin resistance phenotypically. Out of (96) MRSA isolates, only (83) isolates carry mecA and determined MRSA by PCR. A total of (32/115) S. aureus
isolates (27.8%) determined vancomycin resistance phenotypically. Out of (32) VRSA isolates, only (19) isolates carry vanA and determined VRSA by PCR.