Pharmaceutical Standardization and antimicrobial activity of Shila Sindoora

Abstract

Shila sindoora (SS) is a mercury-based ayurvedic formulation. It is indicated in all types of skin disorders, respiratory disorders, abscess and gonorrhea. Present study aims to standardize its standard manufacturing process (SMP) and analytical tests for SS batches. Antimicrobial activity of SS will also be evaluated using microbial strains Staphylococcus aureus (s. aureus), Pseudomonas aeruginosa (p. aeruginosa).  The pharmaceutical procedure of SS was performed as per the ayurvedic pharmaceutical methods. The analytical tests were assessed as per the Ayurvedic pharmacopeia of India (API). The antimicrobial study was conducted using well diffusion method. The average yield obtained is 45.60% and it got deposited in the inner surface of the neck of the bottle (Galastha Kupipakwa Rasayana). X-ray diffraction (XRD) study showed presence of HgS and As₂S₂ in the product. Inductively coupled plasma mass spectrometry (ICP-MS) study showed 21.80% of Mercury, 6.23% of Sulphur and 14.34% of Arsenic in the compound. Average size of particles found to be 767.1 nm and distribution of particles are in between 652.9 nm to 941.2 nm. The antimicrobial study results that SS batches is more efficient against P. aeruginosa in comparison to S. aureus. The present study concludes that standardization of SS along with its antimicrobial effects are established.