Antimicrobial and Antibiofilm activities of Sea Buckthorn essential oil against E. coli, E. faecalis and St. mutans

Abstract

Introduction: Sea buckthorn (Hippophae rhamnoides) is a plant found throughout Europe and Asia. It produces orange-yellow berries, which have been used over centuries as food, traditional medicine and skin treatment. Escherichia coli is a gram-negative bacillus known to be a part of normal intestinal flora but can also be the cause of intestinal and extra intestinal illness in humans. Enterococcus faecalis is a Gram-positive commensal member of the gut microbiota and is commonly detected in acute periodontitis. As a major etiological agent of human dental caries, Streptococcus mutans lives primarily in biofilms on the tooth surfaces, the so-called dental plaque. Materials and Methods: Subcultures were grown on MHA, MIC and Anti biofilm Activity were analyzed and subjected to elisa plate reading. Results: The zones of clearance were seen for all three species of bacteria only at the highest concentration of 40 μl. Discussions: Sea buckthorn has a variety of beneficial effects including anti-aging and antioxidant properties. Conclusion: Sea Buckthorn essential oil potentially inhibited the microbial growth and biofilm formation by E. coli, St. mutans and E. Faecalis.