In-vitro study of antimicrobial activity of methanolic extract of Vitis vinifera L against Oral pathogens

Abstract

Introduction: The antibacterial properties of plant-derived compounds and fruits have also been attracting attention, mainly in view of the increased antibiotic resistance both in hospitals and in community-acquired infections.​​ Aqueous extract of raisins were examined in this study for their effects on E.faecalis and S.mutans factors to evaluate the antimicrobial activity. Aim: Aim of this study is to determine the antimicrobial activity of methanolic extract of Vitis vinifera L against Oral pathogens. Materials and methods: The antimicrobial activity of dry grapes was evaluated against S.mutans and E.faecalis. MIC was determined by two- fold dilution. After 24hrs of incubation TTC was added to identify the presence of bacteria by producing color thereby determining the MIC. Results:The lowest concentration level of 1.25mg/ml of raisins has potentially inhibited the growth of E.faecalis and S.mutans. The methanol extraction of raisins shows antimicrobial activity of S.mutans and E.faecalis. Conclusion: From this study it is concluded that both the organisms show only anti-microbial activity and it doesn’t show any anti-biofilm activity.