EVALUATION OF COPPER NANO PARTICLES COATED SEABUCKTHORN FOR WOUND HEALING ON RATS

Abstract

Introduction: Sea buckthorn is known to contain a wide range of bioactive compounds including flavonoids, phenolic compounds, carotenoids, and essential fatty acids. These compounds possess strong antioxidant and anti-inflammatory properties that play an important role in tissue repair. Antioxidants help neutralize reactive oxygen species generated during the inflammatory phase of wound healing, thereby protecting newly formed tissues from oxidative damage. The presence of vitamins such as vitamin C and vitamin E in sea buckthorn also contributes to collagen synthesis and stabilization, which is essential for proper wound healing.

Materials and methods:Fresh sea buckthorn leaves/fruits were collected and thoroughly washed with distilled water to remove dust and impurities. The plant material was shade dried and powdered. The powdered material was extracted using ethanol/distilled water by the Soxhlet extraction method. The extract was filtered and concentrated using a rotary evaporator and stored at 4°C until further use. Results: The copper nanoparticle-coated sea buckthorn group exhibited the highest percentage of wound contraction by day 14 compared to other groups. Histopathological analysis confirmed better tissue regeneration and organized collagen fibers in the treated group. Conclusion: The synergistic combination of copper nanoparticles and sea buckthorn extract may serve as a promising therapeutic agent for wound management. Further studies involving clinical trials and toxicity evaluation are required to confirm its safety and efficacy in humans.