PREPARATION AND CHARACTERISATION OF ALGINATE BASED COMPOSITE BIOINK FOR BONE TISSUE ENGINEERING APPLICATIONS

Abstract

INTRODUCTION: Three dimensional (3D) printing,a revolutionary approach of regenerating the defective tissue or organ using tissue engineering principles with the help of a component called bioink developed using the naturally available polysaccharides such as alginate has led to the successful regeneration of the bony defect in the field of periodontics.The current study aims at analyzing the compression stability,Surface properties via SEM analysis,biocompatibility and swelling/degenerative capacity of alginate based composite bioink and also aims at fabricating a successful 3D tissue for repair using the same component. MATERIALS AND METHOD: In the current study Alginate Methacrylate (AlgMA) was synthesized using methacrylic anhydride as described by Chou et al and 3D printing ink was fabricated by preparing a homogeneous solution of Resin and PEGDA in ratio 1:1. RESULTS: The Alginate Methacrylate HAP Incorporated Bioink has shown good mechanical structural integrity conductive for cell adhesion,differentiation and proliferation.It is also Biocompatible and shows good cell viability. CONCLUSION: The Novel Alginate Based Composite Bioink developed in the current study has shown good potential properties required for periodontal regeneration of the bony defect using bone tissue engineering applications.