Optimization of Quercetin-Gold nanoparticles synthesis and its antimicrobial effects on skin infection causing microorganisms

Abstract

Introduction: Flavonoids are a class of secondary plant phenolics with significant pharmacological activities that have been studied long ago. Among the flavonoids, quercetin (3,5,7,3′-4′-pentahydroxy flavone) is one of the main flavonoids in the human diet and is mainly found in a glycosylated form (quercetin-3-glucoside) in foods. Several studies have indicated pharmacological effects, such as antioxidant activity, anti-inflammatory, anti-proliferative and anti-angiogenic, anti-ageing, hepatoprotective effect, and renoprotective effects.

Aim: To study the Optimization of Quercetin-Gold nanoparticles synthesis and its antimicrobial effects on skin infection causing microorganisms

Materials and method:

1.Synthesis and characterization of gold nanoparticles with quercetin

1. Characterization by UV–Visible spectroscopy
2. Characterization by FTIR analysis

4.Characterization by transmission electron microscopy (TEM) 5.Evaluation of the stability by the zeta potential method 6.Freeze-drying of the nanoparticles

7.Entrapment efficiency

Discussion:The UV–Vis scanning spectrum showed that AuNPsQct demonstrate absorption bands between 500 and 600 nm, which can be attributed to localized surface plasmon resonance.The infrared spectra recorded similar characteristics, albeit with a lower intensity of the quercetin molecules present after the synthesis of the AuNPs, which occurred through irreversible electrostatic interactions.Regarding the stability of the AuNPs, their relationship is directly proportional to the zeta potential (ZP), which reflects the surface potential of the particles, which is influenced by the changes in the interface with the dispersing medium, due to the dissociation of the functional groups in the surface of the particle or the adsorption of the ionic species present in the aqueous medium.