Pharmacognostic, Phytochemical and Pharmacological Validation of Selected Medicinal Plants: Acorus calamus, Mucuna pruriens, Moringa oleifera and Coffea Arabica
DOI:
https://doi.org/10.7492/gpsrzn41Keywords:
Pharmacognosy, phytochemical screening, HPTLC, herbal, standardization, medicinal plantAbstract
Medicinal plants are an important source of bioactive compounds used in traditional and modern medicine. The present study focuses on the pharmacognostic and
phytochemical validation of selected medicinal plants, namely Acorus calamus, Mucuna pruriens, Moringa oleifera, and Coffea arabica. Pharmacognostic
evaluation was carried out through macroscopic, microscopic, and powder analysis to establish diagnostic features for identification. Physicochemical parameters
such as ash values, moisture content, and extractive values were determined for standardization. Preliminary phytochemical screening was performed to detect the
presence of major secondary metabolites. High Performance Thin Layer Chromatography (HPTLC) was used to develop fingerprint profiles and identify marker
compounds. The results confirmed characteristic morphological features and the presence of phytoconstituents such as alkaloids, flavonoids, tannins, and
terpenoids. HPTLC analysis revealed distinct bands corresponding to β-asarone, L-DOPA, quercetin, and caffeine. The study provides scientific validation for
authentication and quality control of these medicinal plants
