IDENTIFICATION OF B CELLS EPITOPES FROM HSP60 GENES OF HELICOBACTER PYLORI FOR THE POTENTIAL DEVELOPMENT OF ENZYME IMMUNOASSAY

Abstract

Helicobacter pylori H pylori a microaerophilic spiral shaped Gram negative bacterium is a group I carcinogen since it infects the stomachs of over half of the worlds population. Antigenic area or B cell epitope refers to a portion or region of an antigen that is recognised by a particular antibody or B cell. The aim of our study is to identify B cell epitopes from the HSP60 gene of Helicobacter pylori for the potential development of enzyme immunoassay. The amino acid sequence of the transmembrane protein of H pylori was retrieved from the NCBI database and UniProt ID FN598874. The sequence was utilized to identify potential immunogenicity B cell epitopes using the IEDB analysis resource online server program. The listed epitopes were tabulated and analyzed. Epitopes of length 15 to 25 mers were selected. Peptides GYLSPYFVTNAEKMTAQ and VISEELGLSLENAEVEFLGK are suitable for the potential development of B cell epitope peptide vaccines and for the development of indigenous antigen based enzyme immunoassay. Through this study we identified the peptides GYLSPYFVTNAEKMTAQ and VISEELGLSLENAEVEFLGK with the potential for peptide vaccine development against H pylori and for the development of a specific enzyme linked assay.