CHITOSAN ZINC STRONTIUM COMPOSITE MEMBRANE FOR BONE REGENERATION APPLICATION

Abstract

Bone regeneration which may be observed during typical fracture healing and is involved in continual remodelling throughout adult life is a complicated well orchestrated physiological process of bone creation. Avascular necrosis atrophic non unions and osteoporosis are a few complex clinical conditions in which large amounts of bone regeneration are needed such as for the skeletal reconstruction of large bone defects caused by trauma infection tumour resection and skeletal abnormalities or situations in which the regenerative process is impaired. Chitosan Cs from Shrimp Shells degree of deacetylation 75 percent and collagen Col Type I Insoluble from Bovine Achilles Tendon were purchased from Sigma Aldrich. Hydroxyapatite HA was synthesized in our laboratory following the reaction 10Ca OH2 plus 6 NH4 2HPO4 gives Ca10 PO4 6 OH2 plus 12NH4 OH plus 6H. Chitosan Collagen composite membranes Cs Col were obtained varying collagen content into the chitosan solution. Briefly collagen 0.15 and 0.75 percent w v was dispersed into a chitosan solution prepared as described above by mechanical stirring until a good collagen dispersion was obtained. Then 25 g of solution was poured into Petri dishes and dried at room temperature. The membranes with the highest hydroxyapatite and collagen content showed the highest cell adhesion and no cytotoxicity was presented by any of the membranes prepared suggesting that these materials have a significant potential to be used for bone regeneration.