Isolationand identification ofMultidrug-Resistant(MDR) bacteria from wastewater in some hospitals in Basrah

Abstract

Wastewater samples were collected for some hospitals and nearby houses in Basra Governorate. Fifteen samples of wastewater were collected
from some hospitals in Basra Governorate. Two samples were taken from each hospital, including wastewater, before and after
treatment. An effluent water sample was taken from the homes near these Hospitals ,and then 47 bacterial isolates were isolated from
these samples and, The results showed that the number of bacterial isolates before sewage water treatment at a percentage of ( 44.68%)
, the number of bacterial isolates after sewage water treatment at a percentage of ( 34.04%) nd the number of bacterial isolates samples
from the effluent water from a domestic house near a hospital house at a percentage of( 21.27%). growth-negative isolates at a percentage
93.61 % and growth-positive samples isolates at a percentage (6.38%). 16 samples were diagnosed with multidrug resistance (MDR) at a
percentage (34.04%) of the total isolates were positive and negative in growth bacterial sample , represented by Burkholderia Ciencia, (2.12%),
Pseudomonas aeruginosa. (10.63%), Klebsella Pneumoniumea,( 8.51%), Staphylococcus aureus, (4.25%), Enterobacter aerogenes and
Staphylococcus Pseudintermedius by (2.12%), Escherichia coli (4.25%). The results of the study showed that all isolates Gram-positive of were
characterized by their resistance to multiple antibiotics such as Trimethoprim, Benzylpenicillin, and Oxacillin; the current study also showed the
resistance of the Gram-negative bacterial isolates to Tetracycline, Trimethoprim/ Sulfamethoxazole, Gentamicin, Aztreonam, Oxacillin
Amoxicillin , Doxycycline. The results also showed the sensitivity of all Gram- positive isolates to the Ciprofloxacin, Gentamicin, Vancomycin
antibiotics. At the same time, the results also indicated that some Gram-negative bacterial isolates are sensitive to Piperacillin, Meropenem,
Ciprofloxacin and Gentamic antibiotics.